Xenograft tissues contain primary cilia. A-C: Indirect immunofluoresence for cilia components. Adenylyl cyclase III (green) indicates cilia shafts, rootletin (red) indicates the basal body at the base of the cilia and Hoechst dye (blue) indicates cell nuclei. A: Cilia are present in both epithelial and dermal cells. B: Zoom of epithelial cells in A. C: Zoom of dermal cells in A. D: Indirect immunofluoresence for cilia components and suprabasal cells. A cyclase III (green) and rootletin (red) mark cilia. Keratin10 (blue) marks suprabasal cells. Hoechst dye (white) indicates cell nuclei. Robust cilia are present only in cells excluding keratin 10. D': Zoom of boxed areas in D. All dashed lines indicate epidermal/dermal boundaries.
DeRouen et al. BMC Developmental Biology 2010 10:112 doi:10.1186/1471-213X-10-112