Morphological alterations induced by ectopic ΔNp53 expression are partially rescued by knock-down of p21 expression and completely rescued by knock-down of FLp53. (A) Quantitative RT-PCR analysis of steady-state p21 and Δ113p53 transcript levels at 30 hpf in unirradiated embryos microinjected with 1 ng/ml of mRNAs as indicated. (B) Quantitative RT-PCR analysis of steady-state p21 and Δ113p53 transcript levels in irradiated (20 Gy at 24 hpf) embryos harvested at 30 hpf. (C) Representative images of whole embryos (48 hpf) injected with 1 ng mRNA encoding ΔNp53 and MOs targeting either p21  or FLp53  or standard MO control (GeneTools). MO solutions (1 mM) were prepared in sterile water, diluted 3:1 (v:v) with Phenol Red, and 4 nL injected into 1-2 cell embryos.
Davidson et al. BMC Developmental Biology 2010 10:102 doi:10.1186/1471-213X-10-102