Overexpression of miRNAs reduces predicted target mRNA levels. Pools of miR-124, miR-125 and miR-9 miRNA mimics (miRNAs) or scrambled controls (Scrambled) were transfected into HEK293 cells. The mRNA expression of six genes predicted to be targeted by these three miRNAs was subsequently assessed by qRT-PCR. The graph shows the significant (P < 0.01) reduction in expression of ACCN2, ETS1, KLF13, LIN28B and SH2B3 following transfection with miRNAs relative to the scrambled control (error bars show standard deviation). There was no significant difference in expression of NFIB.
Arora et al. BMC Developmental Biology 2010 10:1 doi:10.1186/1471-213X-10-1