Table 1

Nuclear swelling and polar body formation at 15 h after fusion in porcine embryos reconstructed by transferring confluent (CON) or nocodazole-treated (NOC) cells into enucleated M I oocytes







No. of embryos with














Condensed chromatin
















No. of


No


One &

Swollen nucleus












No. of
complexes
No. of embryos
chromatin


polar
Total


Total












Donor
trials
Treated
Fused (%)a
Examined
Surviving
/nucleus
One
Two
body (%)b
(%)b
One
Two
(%)b

CON
3
128
77 (60.2)c
40
40
4
34
0
0(0)
34 (85.0)c
2
0
2 (5.0)
NOC
4
248
64 (25.8)d
32
30
10
9
6
4(13.3)
19 (63.3)d
0
1
1 (3.3)

aPercentage of complexes treated. bPercentage of embryos surviving. c-dValues with different superscripts are significantly different (P < 0.05).

Miyoshi et al. BMC Developmental Biology 2001 1:12   doi:10.1186/1471-213X-1-12