|
Nuclear swelling and polar body formation at 15 h after fusion in porcine embryos reconstructed by transferring confluent (CON) or nocodazole-treated (NOC) cells into enucleated M I oocytes |
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| No. of embryos with |
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|
|
|||||||||||||
| Condensed chromatin |
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|
|
|||||||||||||
| No. of |
No |
One & |
Swollen nucleus |
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|
|
|||||||||||||
| No. of |
complexes |
No. of embryos |
chromatin |
polar |
Total |
Total |
|||||||
|
|
|
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| Donor |
trials |
Treated |
Fused (%)a |
Examined |
Surviving |
/nucleus |
One |
Two |
body (%)b |
(%)b |
One |
Two |
(%)b |
|
|
|||||||||||||
| CON |
3 |
128 |
77 (60.2)c |
40 |
40 |
4 |
34 |
0 |
0(0) |
34 (85.0)c |
2 |
0 |
2 (5.0) |
| NOC |
4 |
248 |
64 (25.8)d |
32 |
30 |
10 |
9 |
6 |
4(13.3) |
19 (63.3)d |
0 |
1 |
1 (3.3) |
|
aPercentage of complexes treated. bPercentage of embryos surviving. c-dValues with different superscripts are significantly different (P < 0.05). | |||||||||||||
Miyoshi et al. BMC Developmental Biology 2001 1:12 doi:10.1186/1471-213X-1-12 |
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