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Intracellular localization of p49 protein in cultured H9C2 cells
. Image analyses was performed on more than 200 H9C2 cells transfected with p49-GFP
at 1,2 and 3 days after transfection. Cytosolic and nuclear signal intensity of p49
was computed using "Image J" program (see description in "Methods").
: No significant relationship was found between p49-GFP cytosolic fraction and day
post-transfection(Day, x axis).
: Images of cells with p49-GFP evident in the extranuclear region were measured after
subtracting the background noise. The images were grouped based on the number of days
post-transfection. In our studies, cell growth displayed a negative quadratic function
during days 1–3 post-transfection (R2adj = 0.273, regression not shown).
Zhang et al. BMC Cell Biology 2008 9:8 doi:10.1186/1471-2121-9-8
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