RT-PCR in untransfected HEK293 cells (HEK293), HEK293 cells transiently transfected with pNBC1 (+pNBC1), as well as human kidney and colon samples using kNBC1- and pNBC1-specific primers (see methods section). While neither isoform was detected in untransfected HEK293 cells, a pNBC1 fragment of the expected size (612 bp) was amplified from transfected HEK293 cells and human colon. kNBC1 was exclusively detected in human kidney samples (expected PCR product size: 489 bp). H2O indicates the reaction where water was used as a template.
Bachmann et al. BMC Cell Biology 2008 9:70 doi:10.1186/1471-2121-9-70