Table 1

RNAi – medited knockdown of CK18 inhibits intracellular multiplication of amastigotes of Y or CL-Brener strains

Parasite Strain

% of infected cell

Amastigotes/100 cells


Medium

CK18 RNAi a

CTR RNAi a

Medium

CK18 RNAi a

CTR RNAi a

Y

32.0 ± 4.95

30.0 ± 3.53

28.0 ± 2.83

578 ± 11.36

142 ± 20.66b

497 ± 16.04

CL-Brener

33.0 ± 2.0

35.0 ± 4.16

33.0 ± 4.04

411 ± 14.57

176 ± 18.55b

406 ± 16.92


a HeLa cells were transiently transfected with CK18 or control RNAi 24 h earlier as described in Methods section. These cells were infected with culture trypomastigotes of Y or CL-Brener strain using the parasite per cell ratio of 10:1 or 5:1 respectively. The number of intracellular amastigotes was estimated 48 h post-infection and the results are expressed as average of triplicates ± SD. This experiment was performed 3 times with similar results.

b The number of amastigotes in cultures containing medium or RNAi control are lower than the number of amastigotes in cultures containing Medium with control RNAi (P < 0.01 in all cases, One Way Anova).

Claser et al. BMC Cell Biology 2008 9:68   doi:10.1186/1471-2121-9-68

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