Additional file 2.
Effects of different concentrations of VPA on morphological differentiation and proliferation of NPCs. NSCs were isolated from rat cerebral cortex at embryonic day 14 (E14) and cultured in N2 medium supplemented with 10 ng/ml bFGF as described in Methods. At 40% confluence, the cultures were treated with 0, 0.5, 1, 2, 3, or 10 mM VPA and incubated for an additional 48 h. Micrographs were taken using a Nikon ECLIPSE TE2000-U fluorescence microscope at 200× magnification.
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Jung et al. BMC Cell Biology 2008 9:66 doi:10.1186/1471-2121-9-66