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Open Access Highly Accessed Research article

Arp2/3 complex activity in filopodia of spreading cells

Simon A Johnston1, Jonathan P Bramble4, Chun L Yeung2, Paula M Mendes2 and Laura M Machesky13*

Author affiliations

1 University of Birmingham School of Biosciences, Edgbaston, Birmingham, B15 2TT, UK

2 Department of Chemical Engineering, Edgbaston, Birmingham, B15 2TT, UK

3 CRUK Beatson Institute for Cancer Research, Garscube Estate, Switchback Rd, Bearsden, Glasgow, G63 9AE, UK

4 Molecular and Nanoscale Physics Group, School of Physics and Astronomy, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK

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Citation and License

BMC Cell Biology 2008, 9:65  doi:10.1186/1471-2121-9-65

Published: 9 December 2008

Abstract

Background

Cells use filopodia to explore their environment and to form new adhesion contacts for motility and spreading. The Arp2/3 complex has been implicated in lamellipodial actin assembly as a major nucleator of new actin filaments in branched networks. The interplay between filopodial and lamellipodial protrusions is an area of much interest as it is thought to be a key determinant of how cells make motility choices.

Results

We find that Arp2/3 complex localises to dynamic puncta in filopodia as well as lamellipodia of spreading cells. Arp2/3 complex spots do not appear to depend on local adhesion or on microtubules for their localisation but their inclusion in filopodia or lamellipodia depends on the activity of the small GTPase Rac1. Arp2/3 complex spots in filopodia are capable of incorporating monomeric actin, suggesting the presence of available filament barbed ends for polymerisation. Arp2/3 complex in filopodia co-localises with lamellipodial proteins such as capping protein and cortactin. The dynamics of Arp2/3 complex puncta suggests that they are moving bi-directionally along the length of filopodia and that they may be regions of lamellipodial activity within the filopodia.

Conclusion

We suggest that filopodia of spreading cells have regions of lamellipodial activity and that this activity affects the morphology and movement of filopodia. Our work has implications for how we understand the interplay between lamellipodia and filopodia and for how actin networks are generated spatially in cells.