Immunofluorescent staining of alpha-tubulin and acetylated tubulin in ARPE-19 cells. ARPE-19 cells transfected with control siRNA or I-2 siRNA were fixed and triple stained for acetylated-tubulin (orange), DNA (blue) and alpha-tubulin (green). I-2 knockdown cells (C and D) showed reduction in acetylation of axonemal tubulin compared with control cells (A and B), without change in the staining of alpha-tubulin. Scale bar = 25 micron.
Wang and Brautigan BMC Cell Biology 2008 9:62 doi:10.1186/1471-2121-9-62