Table 5

FOXO binding sites confirmed by chromatin immunoprecipitation.

Gene

Binding site position

TRANSFAC 8.4

TRANSFAC 11.1

Primer Position


ATROGIN-1

-31

V$HFH8_01

-160

-118

V$FOXO1_01

V$HFH1_01

-136

V$FOXO1_01

V$FOXO1_01


BCL6

-837

V$HFH1_01

-1126

-881

V$FOXO1_02

-991

V$FOXJ2_01

V$HFH1_01

-1419

V$HFH1_01


BTG1

-213

V$FOXJ2_01

V$FOXO1_01

-254

-220

V$HFH1_01

-496

V$FOXO1_01

V$HFH1_01


CCNG2

-158

V$FOXO1_01

V$HFH1_01

-243

-165

V$FOXO1_01

-469

V$FOXO1_01

-473

V$FOXO1_01

V$FOXO3_01

-483

V$FOXO1_01


DDIT3/CHOP

-1544

V$FOXO1_01

-1973

-1606

V$HFH1_01

-1830

V$FOXO1_02

-2069

V$FOXO1_01


K1F1B

-673

V$FOXO1_01

-673


KLF6

-955

V$FOXO1_01

V$HFH1_01

-947

-929

V$FOXO3_01

-887

V$FOXJ2_01

V$HFH1_01


KLHL24

-14

V$FOXO3_01

-211

-34

V$FOXO1_01


TXNIP

-216

V$FOXO1_01

V$HFH1_01

-250


ZBTB1

-252

V$FOXO1_02

-263


The predicted FOXO binding sites that were confirmed by ChIP are indicated by the most 5' nucleotide. When multiple matrices predicted overlapping sites, the position indicates the most 5' nucleotide of the consensus sequence (see Additional file 4 for all sequences). The TRANSFAC matrices shown are those that predicted the indicated sites with the lowest p-values. ChIP assays were considered to confirm binding sites located within 500 bp of the primer position. Primer positions are indicated by the most 5' nucleotide of the forward primer; all amplicons were 50–60 bp.

Terragni et al. BMC Cell Biology 2008 9:6   doi:10.1186/1471-2121-9-6

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