PI 3-kinase inhibition causes a decrease in RelB binding. T98G cells were either maintained in serum or treated with 50 μM LY294002 for 4 or 8 hours. Chromatin fragments were immunoprecipitated with anti-RelB antibody and quantified by real-time PCR. Data are presented as the percentage of input and are the means of 3 independent experiments ± S.E. β-globin was used as the negative control. (*) represents statistically significant loss of binding compared to the cells maintained in serum (assessed by t test).
Terragni et al. BMC Cell Biology 2008 9:6 doi:10.1186/1471-2121-9-6