Effect of PI 3-kinase inhibition on T98G cells. A, Cell extracts were harvested from starved T98G cells that were rendered quiescent by 72 hours of incubation in serum-free medium, from actively proliferating T98G cells in serum-containing medium, and from quiescent cells that had been stimulated by treatment with 20% serum or PDGF for 30 minutes. Extracts were subjected to SDS-PAGE and immunoblotted with anti-phospho Akt and pan anti-Akt antibodies. B, Actively proliferating T98G cells in serum-containing media were treated with either 50 μM LY294002 or 0.1% DMSO (vehicle control, 0 minute timepoint). Cell extracts were analyzed by immunoblotting. C, Proliferating T98G cells were treated with LY294002 for the indicated times. Cytosolic nucleic acids were isolated and DNA fragmentation was assessed by gel electrophoresis. D, Proliferating T98G cells were harvested at the indicated times after LY294002 treatment, subjected to TUNEL assay, and TUNEL-positive cells quantified by flow cytometry.
Terragni et al. BMC Cell Biology 2008 9:6 doi:10.1186/1471-2121-9-6