The assembly of aggresomes or dispersed aggregates is independent of the level of expression of CyPrPEGFP. Aggregates were visualized by fluorescence 24 h after addition of different concentrations of tetracycline in the culture medium of N2a and Hela cells. From left to right, exposure times for the green channel are 850 ms, 400 ms, and 60 ms. Insets represents western blot analysis of CyPrPEGFP (top band) or tubulin to check for equal loading (bottom band). Results are representative of 3 independent experiments.
Beaudoin et al. BMC Cell Biology 2008 9:59 doi:10.1186/1471-2121-9-59