FIT as a tool to elucidate the role of kinases in a multi-molecular complex. FAK, Src and p130Cas are known to exist in multi-molecular complexes. Since both FAK and Src possess kinase activity, either one (or both) could phosphorylate p130Cas. The importance of Src or FAK as the kinase responsible for phosphorylating p130Cas can be easily studied by using kinase-dead (kd) versions of each. From our results, we conclude that the Src kinase activity is responsible for phosphorylating p130Cas.
Sharma and Mayer BMC Cell Biology 2008 9:50 doi:10.1186/1471-2121-9-50