Tyrosine phosphorylation in SYF cells is increased by expressing c-Src or v-Src, but not by FIT-compatible Src. SYF cells were transfected with plasmids expressing GFP-actin alone (A) or in combination with v-Src (C) or FIT-compatible Src (D) while c-Src reconstituted cells were transfected with GFP-actin vector only (B). GFP-actin and anti-pTyr immunofluorescence were observed by confocal microscopy of fixed, permeabilized cells. Merged image is presented on right. Scale bars represent 10 μM.
Sharma and Mayer BMC Cell Biology 2008 9:50 doi:10.1186/1471-2121-9-50