Additional file 1.
p34 (ARPC2) localisation during the exit of mitosis. A. HeLa cells were enriched in mitosis after thymidine and RO3306 blocks (see Methods for details). Two hours after release from RO3306 block cells were permeabilised and fixed as described in Methods. Cells were treated for indirect Alexa 555 localisation of p34 (a and e) and Alexa 350 localisation of β-tubulin (c and g) with specific antibodies. F-actin was visualised with FITC-coupled phalloidin (b and f). Merged images are presented (d and h). Images are representative of the different stages of mitosis: late telophase (a-d) and cytokinesis (e-h). During late telophase p34 is enriched within the contractile ring (d, arrowhead) During cytokinesis, p34 presented a vesicular-like staining at the basis of microtubule bundles (e, arrowhead) and was found within the cytoplasmic bridge between the two daughter cells (e, arrow). Colocalisation with F-actin was also evident at the cortex facing the cytoplasmic bridge (h, arrowhead). B. Arp3 and p34 localisations are undistinguishable HeLa cells prepared as previously described were treated for indirect Alexa 555 localisation of Arp3 (a) and Alexa 488 localisation of p34 (b) with specific antibodies. DNA was stained with DAPI. A merged imaged is presented (d) and highlight the strong colocalisation between the two Arp2/3 complex subunits in particular within the cleavage furrow. Bars, 10 μm.
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Bompard et al. BMC Cell Biology 2008 9:42 doi:10.1186/1471-2121-9-42