Wiskostatin inhibits late cytokinesis. A. Wiskostatin does not affect microtubule organisation. HeLa cells stably expressing GFP-tagged β-tubulin were synchronised in prometaphase with nocodazole and released after a shake-off in presence of vehicle (Cont) or 10 μM wiskostatin (Wisko). 120 minutes after release cells were fixed and F-actin (a and e) and DNA (c and g) stained respectively with Alexa 555-coupled phalloidin and DAPI. β-tubulin (b and f) was directly visualised. Merged images (d and h) are presented. B. Wiskostatin alters Arp3 localisation. HeLa cells were synchronised in G2/M by thymidine and RO3306 double block. 120 minutes after release cells were fixed and stained as described in Figure 1. Images a and e represent staining of Arp3, b and f staining of F-actin and c and g staining of β-tubulin. Merged images (d and h) are presented. Bars, 10 μm.
Bompard et al. BMC Cell Biology 2008 9:42 doi:10.1186/1471-2121-9-42