Lysine 36 but not lysine 37 of PARP-2 is critical for nuclear localization. A) HA-tagged wild type (wt) PARP-2 or the indicated single mutants were expressed in HEK293T cells and expression was analyzed by western blot using a monoclonal anti-HA antibody. 50 μg of whole cell extracts were used, endogenous PARP-1 levels served as loading control. B) HEK293T cells were transfected with HA-tagged wild type (wt) PARP-2 or with the PARP-2 mutants K36R and K37R. HA-tagged proteins were detected by immunofluorescence as described for Figure 2A. Representative images are presented.
Haenni et al. BMC Cell Biology 2008 9:39 doi:10.1186/1471-2121-9-39