Figure 7.

Role of the cytoplasmic region in the lateral mobility of paranodin analyzed by FRAP. (A) Images of neuroblastoma N2a cells co-transfected with paranodin-Δcyto (pndΔcyto) and contactin showing an example of a FRAP sequence. Bar: 5 μm. Kinetics of fluorescence recovery of GFP-tagged paranodin (B) and paranodinΔcyto (C). Data are normalized to yield an intensity of zero immediately after photobleaching and represents the average of 8 individual traces (mean ± SEM). Deletion of the cytoplasmic tail of paranodin significantly (P < 0.05) increased the mobile fraction of paranodin-GFP as assessed using ANOVA.