Figure 3.

FET proteins localize to stress granules. Images show FET proteins (green) and immunostaining for the stress granule marker TIA-1 (red). Nuclei are visualized by DAPI staining (blue) in the bottom image of each column (a) Overexpression of FET-GFP proteins cause stress granule formation in transiently transfected HT-1080 cells. (b) HT-1080 cells stably expressing FET-GFP proteins show localization of tagged proteins to stress granules upon arsenite treatment. (c) Endogenous FET proteins localize to stress granules in response to oxidative stress in HeLa cells. (d) Stable transfectants of FUSA-GFP and GFP show similar minor signal in granules upon arsenite treatment. Scale bars indicate 5 μm.