The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response

doi:10.1186/1471-2121-9-37

BMC Cell Biology
Volume 9

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Andersson MK
Ståhlberg A
Arvidsson Y
Olofsson A
Semb H
Stenman G
Nilsson O
Åman P

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Andersson MK
Ståhlberg A
Arvidsson Y
Olofsson A
Semb H
Stenman G
Nilsson O
Åman P
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Research article

The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response

Mattias K Andersson¹ , Anders Ståhlberg²^,3 , Yvonne Arvidsson¹ , Anita Olofsson¹ , Henrik Semb³ , Göran Stenman¹ , Ola Nilsson¹ and Pierre Åman¹

¹Lundberg Laboratory for Cancer Research, Department of Pathology, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden

²Center for Brain Repair and Rehabilitation (CBR), Department of Clinical Neuroscience and Rehabilitation, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden

³Stem Cell Center, Lund University, Lund, Sweden

author email corresponding author email

BMC Cell Biology 2008, 9:37doi:10.1186/1471-2121-9-37


Published:	11 July 2008

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Additional file 1:

Immunofluorescence and transfections. Immunostaining and transfections of FET proteins. (a) Endogenous and transient FET protein expression in four different cultured cell types with DAPI staining of nuclei (blue). Scale bars indicate 10 μM. (b) Stable expression of FET-EGFP proteins in HT1080 with DAPI staining (blue). Scale bars indicate 10 μM.

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Additional file 2:

Cell area measurements. Clones of stable transfectants were seeded out, stained and imaged as described in Materials and methods. Average cell areas were calculated from 130–200 cells in five images per clone. Error bars show standard error of mean.

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Additional file 3:

Proliferation assay. Western blot showing FET expression in contact-inhibited and actively proliferating F470 cells as well as in serum stimulated and starved HT1080 cells. Beta actin expression is used as a loading control.

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Primary antibodies. Primary antibodies used.

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Additional file 5:

Primer sequences. Primers used for cDNA cloning and quantitative real-time PCR.

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