Myocyte elongation is impaired by kindlin-2 knockdown. A. C2C12 cells were transfected with RNAi and either maintained in Growth Media (GM) or changed to Differentiation Media for two days (DM D2). Cellular area was determined using Metamorph on immunostained cells overexposed to reveal their cell borders. There was a small but significant difference between conditions in undifferentiated cells (kindlin-2 RNAi vs. scrambled RNAi: 7692 +/- 172 pixels v 9080 +/- 348 pixels, n= 240 cells, p = 0.006). There was a substantial difference between conditions in differentiated cells (kindlin-2 RNAi vs. scrambled RNAi: 7510 +/- 599 pixels v 13180 +/- 906 pixels, n = 270, p = 0.001). B. C2C12 cells at differentiation day 2 immunostained with anti-kindlin-2. The photomicrographs emphasize the obvious size difference between scrambled RNAi transfected cells and those transfected with kindlin-2 RNAi. They also reinforce the successful knockdown of kindlin-2 protein by RNAi. (scale bar = 20 μm).
Dowling et al. BMC Cell Biology 2008 9:36 doi:10.1186/1471-2121-9-36