RNAi reduces kindlin-2 levels. A. Quantitative PCR analysis of C2C12 cells treated with kindlin-2 or scrambled siRNA. Samples for analysis were collected 1 day (GM), 3 days (DM day 2) and 5 days (DM day 4) after transfection. Relative kindlin-2 levels were (si scrambled vs. si mig2): 33% vs. 8% (GM), 100% vs. 28% (DM day 2), and 83% vs. 36% (DM day 4). Numbers reflect percent level as compared to scrambled RNAi at DM day 2. Values were averaged from 4 independent transfections, each analyzed in triplicate. B. Immunoblot of kindlin-2 on protein extracts obtained after 2 days in DM (72 hrs after transfection). A band corresponding to kindlin-2 (arrow) is observed in the scrambled RNAi lane only. Equal loading was confirmed by reprobing the blot with S6 kinase (S6K) and GAPDH (data not shown).
Dowling et al. BMC Cell Biology 2008 9:36 doi:10.1186/1471-2121-9-36