Phagosome integrity. In A, discrimination of free bacteria from intact and broken phagosomes is illustrated. Alexa 488-labeled annexin V (green) stains phagosomal membrane and Cy3-labeled anti-human Fab fragments (red) stains damaged phagosomes and free opsonized bacteria. Arrows indicate free bacteria, arrowheads intact phagosomes, and the double arrowhead shows a partial phagosome; scale bar 10 μm. B shows quantification of phagosomes from three separate experiments ± SEM.
Lönnbro et al. BMC Cell Biology 2008 9:35 doi:10.1186/1471-2121-9-35