Recruitment of NFAT to the FasL promoter region. ChIP analysis of the FasL promoter region was carried out on cells treated with CORT. The chromatin DNA selected by anti-NFAT antibody was amplified with FasL specific primers. Lane 1, marker; Lane 2, the input of mLTC-1 cells; Lane 3, the ChIP DNA from mLTC-1 cells untreated with CORT. Lane 4, the ChIP DNA from mLTC-1 cells treated with 100 nM CORT; Lane 5, the ChIP DNA from mLTC-1 cells treated with 100 nM CORT and 100 mg/ml CsA; Lane 6, non-specific IgG; Lane7, non-specific primer.
Chai et al. BMC Cell Biology 2008 9:31 doi:10.1186/1471-2121-9-31