Co-transfection of NFAT expression vector activates FasL promoter. A. Leydig cells were co-transfected with FasL-P272 (0.4 μg) and pcDNA3.1-NFAT (NFAT expression vector, 0.8 μg), or empty pcDNA3.1. Luciferase activity was determined after 48 h incubation with CORT. CORT treatment significantly increased the luciferase activity compared with controls. The increase of Luciferase activity in the cells co-transfected with pcDNA3.1-NFAT and FasL-P272 is several times higher than that in the cells cotransfected with pcDNA3.1 and FasL-P272. B. Leydig cells were co-transfected with FasL-P272m (0.4 μg) and pcDNA3.1-NFAT (0.8 μg), or empty pcDNA3.1. Luciferase activity was determined after 48 h incubation with CORT. CORT treatment did not induce elevation of luciferase activity in cells either transfected with or without pcDNA3.1-NFAT. Results represent the average of three independent transfection experiments.
Chai et al. BMC Cell Biology 2008 9:31 doi:10.1186/1471-2121-9-31