Delineation of CORT-responsive cis-acting elements in the FasL promoter. A. Schematic representation of FasL 5' truncation reporter- luciferase constructs. B. A series of FasL-luciferase reporter constructs were transfected into mLTC-1 cells. After overnight incubation, the mLTC-1 cells were treated with 100 nM CORT for an other 12 h. The cells were lysed and assayed for luciferase activity. The luciferase activity was compared with the normalized activity of the transfected but not treated cells. These data are representative of at least three independent experiments and are normalized to an internal Renilla control. ** denote a significant difference compared with control at P < 0.01. * denote a significant difference compared with control at P < 0.05.
Chai et al. BMC Cell Biology 2008 9:31 doi:10.1186/1471-2121-9-31