FRET-efficiency between VHA-e isoforms and subunits VHA-a, VHA-c and VHA-c". CFP-fusion proteins of VHA-e1 and VHA-e2 were expressed in A. thaliana mesophyll protoplasts in combination with YFP-fusion proteins of VHA-a, VHA-c and VHA-c". FRET analysis was performed by confocal laser scanning microscopy (CLSM) to gain information on isoform-specific V-ATPase assembly. The data represent the mean average ± SE (VHA-e1: VHA-a: n = 62; VHA-c: n = 86; VHA-c": n = 40; VHA-e2: VHA-a: n = 36; VHA-c: n = 60; VHA-c": n = 52).
Seidel et al. BMC Cell Biology 2008 9:28 doi:10.1186/1471-2121-9-28