Transcript quantification of VHA-c and VHA-c" isoforms. (A) The transcript levels of VHA-c3 and VHA-c" isoforms were analysed in A. thaliana root and leaf tissue by semiquantitative RT-PCR in the linear range of amplification. Each experiment was performed three times as indicated with 1–3. Actin served as normalisation standard. (B) VHA-c"2 transcript was observed when images of ethidium bromide-stained agarose gels were obtained with increased exposure time (positive control). Using water as template for RT-PCR a PCR-product was not detectable neither for VHA-c"1 nor for VHA-c"2 (negative control). (C) Transcripts of the five VHA-c isoforms were detectable in leaf tissue (positive control), the negative controls using water as templates did not yield the PCR-product (negative control).
Seidel et al. BMC Cell Biology 2008 9:28 doi:10.1186/1471-2121-9-28