Dual-tagged wild type and mutant GLUT4 translocates to the plasma membrane in response to insulin stimulation in 3T3-L1 adipocytes. 3T3-L1 adipocytes were transiently transfected with (a) wild type or (b) 4A mutant GLUT4HA-GFP and serum starved for 4 hr, stimulated with 83 nM insulin for 10 min then fixed for confocal microscopy as described in Materials and Methods. Green staining shows total GLUT4HA-GFP, red denotes surface exposed HA epitope.
Heyward et al. BMC Cell Biology 2008 9:25 doi:10.1186/1471-2121-9-25