Table 2

Primers used for plasmid construction

Primers

Template


cdc13-des2-HBD:

TCCTC

    CATATG
ACTACCCGT

pREP81-cdc13-des2

A CAC TAA A

    TT AAT T
AA CCA TTC

wee1-HBD:

GGAATTC

    CATATG
AGCTCTTCTTCTAATAC

Genomic

C C

    TT AAT TAA
AAC ATT CAC CTG CCA ATC TT

HO-HBD:

GGAATTC

    CATATG
CTTTCTGAAAACACGAC

Genomic

C C

    TT AAT TAA
GCA GAT GCG CGC ACC TGC GT


Bøe et al. BMC Cell Biology 2008 9:23   doi:10.1186/1471-2121-9-23

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