Open Access Methodology article

Rapid regulation of protein activity in fission yeast

Cathrine A Bøe1, Ignacio Garcia3, Chen-Chun Pai2, Jeffrey R Sharom4, Henriette C Skjølberg1, Erik Boye1, Stephen Kearsey2, Stuart A MacNeill3, Michael D Tyers4 and Beáta Grallert1*

Author Affiliations

1 Department of Cell Biology, Rikshospitalet-Radiumhospitalet Medical Centre, Montebello, 0310 Oslo, Norway

2 Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK

3 Department of Molecular Biology, University of Copenhagen, Københavns Biocenter, Ole Maaløes Vej 5, 2200 Copenhagen N, Denmark

4 Samuel Lunenfeld Research Institute, Room 1080, Mount Sinai Hospital, 600 University Avenue, Toronto Ontario, M5G 1X5, Canada

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BMC Cell Biology 2008, 9:23  doi:10.1186/1471-2121-9-23

Published: 5 May 2008

Additional files

Additional file 1:

Limitations of the system. Two examples where fusing the ERHBD tag to the protein of interest did not lead to regulatability with estradiol are presented.

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Additional file 2:

HO-HBD has little HO activity even in the presence of estradiol. The data provided present evidence that the HO-HBD fusion protein retains little endonuclease activity even in the presence of estradiol.

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Additional file 3:

Wee1-HBD is active even in the absence of estradiol. The data provided present evidence that the Wee1-HBD fusion protein is active even in the absence of estradiol.

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