Confocal immunofluorescence analysis of various NP constructs containing the obpNLS. COS-7L cells transfected with the various NP-GFP constructs were fixed and mounted with propidium iodide-containing medium to indicate nuclear staining. NP-L exhibits a typical punctuate, nuclear localization pattern. obpNLS+NC, obpNLS+N-ter and obpNLS+C-ter all localize to the nucleus.
Ketha and Atreya BMC Cell Biology 2008 9:22 doi:10.1186/1471-2121-9-22