Figure 4.

Levels of EGFR protein correlate with levels and activities of Cα and Cβ in HeLa cells. (a) HeLa cells incubated with RNAi against Cα and Cβ and monitored for PKA-specific phosphotransferase activity in cell extracts of 1 mg/mL. Relative values given as counts per minute (cpm). (b) Relative EGFR mRNA expression levels were measured by RT-PCR and are given as E/G × 100 where E and G are the relative copy numbers of EGFR and GAPDH mRNA, respectively. The results are given as +/- SEM where n = 3. (c) Western immunoblotting analysis of expression of EGFR and PKA C. An anti-actin antibody was applied to ensure equal loading.

Oksvold et al. BMC Cell Biology 2008 9:16   doi:10.1186/1471-2121-9-16
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