A novel semi-automatic image processing approach to determine Plasmodium falciparum parasitemia in Giemsa-stained thin blood smears

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Discussion of implementation details with case study. The text illustrates the required processing steps for the semi-automatic approach and provides details based on selected case studies.

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Detection of nucleated components. The illustration depicts the process of detecting nucleated components which comprises the comparison of the different color channels with consecutive thresholding.

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Image decomposition. The illustration addresses the image decomposition in background and solid matter based on the compensation of illumination differences and repetitive thresholding.

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Segmentation process. The illustration visually describes the segmentation of clusters of erythrocytes into individual erythrocytes.

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Assignment of parasites to erythrocytes. The illustration provides examples for infections in detected erythrocyte clusters and addresses the association of a parasite to the correct host cell.

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