Figure 3.

Subcellular localization of VCP/p97 in undifferentiated controls (U937), differentiated populations (3d, 7d, 14d) and retrodifferentiated U937 cells (Retro) by immunofluorescence. A. VCP/p97 was visualized by a secondary FITC-conjugated rabbit anti-goat antibody (VCP-Ab). The nuclei were stained with the DNA-intercalating dye Hoechst 33258. B. The VCP/p97 antibody was neutralized with a specific blocking peptide and incubated with a secondary FITC-conjugated rabbit anti-goat antibody (VCP-Ab + VCP-peptide). The nuclei were stained with the DNA-intercalating dye Hoechst 33258.