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Open Access Highly Accessed Research article

Actin binding domains direct actin-binding proteins to different cytoskeletal locations

Raymond W Washington12 and David A Knecht1*

Author Affiliations

1 Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA

2 Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Ave, RN261 Boston, MA 02215, USA

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BMC Cell Biology 2008, 9:10  doi:10.1186/1471-2121-9-10

Published: 13 February 2008

Additional files

Additional File 1:

Movie showing the association of GFP-FLN with macropinosome. The movie shows the association of GFP-FLN with the forming macropinosome and then its disappearance from the vesicle membrane after internalization.

Format: MOV Size: 264KB Download file

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Open Data

Additional File 2:

Movie showing the association of GFP-FLNABD with macropinosome The movie shows the association of GFP-FLNABD with the forming macropinosome and then its disappearance from the vesicle membrane after internalization.

Format: MOV Size: 189KB Download file

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Open Data

Additional File 3:

Movie showing the association of GFP-aA with macropinosome. The movie shows the association of GFP-aA with the forming macropinosome and then its disappearance from the vesicle membrane after internalization.

Format: MOV Size: 94KB Download file

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Open Data

Additional File 4:

cAMP chemotaxis of AX2 wild type cell expressing GFP-FLN. Dictyostelium AX2 cells were incubated in starvation buffer for 6–8 hrs and images were captured early in the streaming process. The cells had become polarized and directional movement was observed. The GFP-FLN probe is absent from the broad leading lamellae of the chemotaxing cell but is localized to the lateral and rear cortical regions.

Format: MOV Size: 168KB Download file

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Open Data

Additional File 5:

Simultaneous localization of GFP-FLN (green) and mRFP-αA (red) in a polarized cell. An AX2 cell was imaged while chemotaxing up a cAMP gradient. The image clearly shows the differential location of filamin (red) and α-actinin (green) when the cell is polarized. Filamin localizes to the rear of the cell in the cortical area, and α-actinin localizes to the front of the cell and new protrusions, one of which becomes the leading edge of the cell.

Format: MOV Size: 1.9MB Download file

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Open Data