LBF analysis of the distribution of NuMA from 3D images. (a) Fluorescence micrograph of Texas red-immunolabeled NuMA from a single optical section, in differentiated non-neoplastic S1 cells. (b) The corresponding processed image section showing a composite view of the detected local bright features (light gray) of NuMA, extracted by the local bright feature analysis overlaid on the nuclear segmentation mask (dark gray). (c) Concentric terraces resulting from the application of the distance transform on the segmentation mask, which allows the radial distribution of NuMA to be calculated. (d) A set of LBF distribution profiles of NuMA calculated from differentiated non-neoplastic S1 cells. The relative density of NuMA bright features (ordinate) is plotted as a function of the relative distance from the perimeter (0.0) to the center (1.0) of the nuclei (abscissa).
Long et al. BMC Cell Biology 2007 8(Suppl 1):S3 doi:10.1186/1471-2121-8-S1-S3