Colocalization of CLIC1 with megalin and NaPi-II in MPTC cells. MPTC cells were grown on collagen-coated filters, fixed, permeabilized, and stained for CLIC1 with the 9F5 monoclonal antibody (green, center column) and two markers of the apical endocytic/recycling compartment (red, left column). The right column in each set is a merged image generated from the red and green channels. A. Two separate fields from near the apical pole of the cells stained for megalin (red) and CLIC1 (green). B. Two separate field from near the apical pole of the cells stained for NaPi-II (red) and CLIC1 (green). The scale bars represent 10 microns in each set of images.
Ulmasov et al. BMC Cell Biology 2007 8:8 doi:10.1186/1471-2121-8-8