Insulin stimulation of SHSY-5Y and C3A cells infected with adenovirus or baculovirus vectors. Cultured SHSY-5Y (A and C) and C3A (B and D) cells were transduced with adenovirus (AdGFP) at MOI10 and 50 or baculovirus (BacGFP) at MOI1000 and 10000, respectively, and the responsiveness to insulin was determined. Non-infected (No virus) and infected cells were treated with 100 nM insulin for 10 (A and B) and 30 minutes (C and D), respectively. Cells were harvested after incubation for total cellular protein, and ELISA was performed with antibodies specific for Akt phosphorylated on serine 473 and total Akt, respectively.
Andersson et al. BMC Cell Biology 2007 8:6 doi:10.1186/1471-2121-8-6