PKC activator PMA did not induce dispersion. Isolated RPE cells were first treated with 10 μM forskolin for 45' to induce aggregation. Following aggregation, cells were either treated with carbachol (100 nM) or with PMA, a PKC activator. The pigment indices of cells treated with PMA were significantly different from the pigment index of cells treated with carbachol and were not significantly different from the pigment index of cells treated with forskolin, indicating that PMA failed to induce pigment granule dispersion.
Johnson and García BMC Cell Biology 2007 8:53 doi:10.1186/1471-2121-8-53