Figure 2.

Relation to Nuclear Structures. (A) GFP-Esc1p was induced for 5 hr to compare the localization of GFP-tagged escapades to the nucleolus, in a strain (ATY2101) which expresses Sik1p-mRFP. Systematic examination of through-focal series shows that > 90% of escapades contact the perimeter of the nucleus at or immediately adjacent to the mRFP-positive nucleolus. See Fig. S5. (B) Comparison of the localization of GFP-tagged escapades with the spindle pole body, in a strain (ATY3276) which expresses Spc42p-mRFP and has been induced for 5 hr. An overview is given in Fig S6. (C) Comparison of the localization of GFP-tagged karmellae with the nucleolus and the spindle pole body, in a strain (ATY1577) which expresses Sik1p-mRFP and Spc42p-GFP and carries plasmid [pGAL-HMG-CoA Reductase I-GFP]. Karmellae are ER membrane stacks associated with the outer nuclear membrane which result from overexpression of HMG-CoA reductase type1. (D) Comparison of the localization of GFP-tagged escapades with a centromere, in a strain (ATY2098) which expresses a GFP-lac repressor fusion and an insertion of lac operator arrays near CENIV and has been induced for 5 hr. An overeview is given in Fig. S7. (E) Comparison of the localization of GFP-tagged escapades with a telomere, in a strain (ATY2097) which expresses a GFP-lac repressor fusion and carries an insertion of lac operator arrays near telomere XIVL and has been induced for 5 hr. An overview is given in Fig. S8. (F) Comparison of the localization of the GFP-tagged telomere-associated protein, Rap1p, and Htb2p-mRFP-tagged chromatin in ATY3275. Induction was for 5 hr. An overview is given in Fig. S9.

Hattier et al. BMC Cell Biology 2007 8:47   doi:10.1186/1471-2121-8-47
Download authors' original image