Additional file 2.
Ste12 overexpression causes hyper-accumulation of Fus3 in the nuclei of pheromone-treated cells. Wild type cells were transformed with either the Fus3-GFP reporter or the Fus3T180AY182A-GFP reporter, and either the GAL1-STE12 plasmid or an empty vector. Strains were grown to mid-log phase in sucrose medium and galactose was added to a concentration of 2% two hours before pheromone treatment (GAL1 promoter on). The cultures were then split and grown with or without the addition of 12 nM pheromone. Images were acquired from the untreated and treated cultures in 2 hour intervals. The 2 hr, 4 hr, and 6 hr time points indicate time elapsed after galactose induction. (A) untreated wild type cells expressing Fus3-GFP; (B) pheromone-treated wild type cells expressing Fus3-GFP; (C) untreated cells expressing Fus3-GFP and overexpressing Ste12; (D) pheromone-treated cells expressing Fus3-GFP and overexpressing Ste12; (E) untreated cells expressing Fus3T180AY182A-GFP and overexpressing Ste12; (F) pheromone-treated cells expressing Fus3T180AY182A-GFP and overexpressing Ste12.
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Blackwell et al. BMC Cell Biology 2007 8:44 doi:10.1186/1471-2121-8-44