Dose-dependent induction of the BRE-Luc construct by BMPs. C2C12BRA and HepG2BRA cells were added to 96-well plates at 4 × 103 cells per/well and 5 × 103 cells/well, respectively. The reporter cells were incubated with increasing concentrations of the indicated recombinant BMPs in DMEM, 0.1% BSA. After 15 h incubation, BMP activity was assessed by measuring luciferase activity in the cell lysates. (A) BMP-2. (B) BMP-4. (C) BMP-6. (D) BMP-7. Background luciferase level in the control sample (no BMP) was subtracted from each of the experimental values. The results are expressed as relative luciferase units (RLU). Each point represents the mean ± SEM of triplicate wells from 1 representative experiment. Similar results were obtained in two separate experiments.
Zilberberg et al. BMC Cell Biology 2007 8:41 doi:10.1186/1471-2121-8-41