Table 1

Effect of PMA on IL-10 induced suppression of NFATc1

Group

NFATc1 copies per 106 β-actin ± SEM

Relative change in expression compared to RANKL group


CONTROL

8.5 × 103 ± 3 × 103 *

0.006 *

RANKL

1.4 × 106 ± 5 × 105

0

RANKL + IL-10

4.6 × 104 ± 1 × 104 *

0.032 *

RANKL + IL-10 + PMA

7.9 × 105 ± 9 × 104

0.56


Quantitative RT-PCR analysis of NFATc1 mRNA expression in RAW264.7 cells. Cultures were treated with RANKL, RANKL and IL-10 or RANKL, IL-10 and PMA for 24 hours and total RNA extracted. Quantitative PCR was performed on a Techne Quantica PCR machine using the DNA binding dye SYBR-green and specific primers for murine NFATc1 and β-actin. For each sample the NFATc1 copy number was expressed as the relative copy number normalised to 106 β-actin copies. Samples were analysed in triplicate and are expressed as the mean of three separate experiments. * p < 0.05 versus RANKL group.

Evans and Fox BMC Cell Biology 2007 8:4   doi:10.1186/1471-2121-8-4

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