Table 1 |
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|
Percent radioactivity recovery in cellular fractions from (1-14C) glucose labeled MCF7 cells |
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|
Lipid-phase |
Inter-phase |
TCA |
Ethanol |
Aqueous |
|
|
|
|||||
|
Zero Incubation |
0.8 |
5 |
6 |
0.02 |
88 |
|
Full Incubation |
0.3 |
6 |
10 |
0.07 |
85 |
|
Transform ation |
0.5 ± 0.2 |
12 ± 3 |
21 ± 3 |
0.4 ± 0.2 |
66 ± 6 |
|
|
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|
The lipid-phase is the bottom fraction from the chloroform:methanol extraction of the cells. The inter-phase contains cell debris and proteins, and the TCA fraction contains proteins from precipitation of the aqueous fraction. The ethanol fraction contains carbohydrates from the remaining aqueous fraction in 5 volumes of ice-cold ethanol incubated at -20°C over night. The remaining water-soluble fraction contains unused 14C-glucose. |
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|
Wu et al. BMC Cell Biology 2007 8:36 doi:10.1186/1471-2121-8-36 |
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