Ubiquitin and proteasome colocalization to aggregates is affected by Ubc knockdown. Antibodies to ubiquitin and a proteasome subunit were used to test the effects of Ubc RNAi. Yellow color in the Merge columns indicates colocalization with aggregates. In control worms, ubiquitin and proteasomes colocalize to polyglutamine aggregates (top row). RNAi with ubq-1 eliminates localization of both ubiquitin and proteasomes. RNAi with rpt-1 does not disrupt localization of ubiquitin to aggregates, but does remove proteasomes, as expected. RNAi with either ubc-1, ubc-13, or uev-1 diminishes localization of ubiquitin and proteasomes to aggregates, whereas ubc-2 and ubc-22 show no effect on colocalization.
Howard et al. BMC Cell Biology 2007 8:32 doi:10.1186/1471-2121-8-32