Immunoprecipitation of tubulin from Hydra lysate with anti-14-3-3 antibody. Western blot stained with anti-tubulin antibody. Lane 1 shows tubulin precipitated with anti-14-3-3 antibody from hydra lysates, in lane 2 the anti-14-3-3 antibody was omitted as control for non-specific binding of proteins to the beads, in lane 3 peptide C was added before precipitation with anti-14-3-3-antibody and in lane 4 the lysate was treated with λ-phosphatase before preciptiation with anti-14-3-3 antibody.
Pauly et al. BMC Cell Biology 2007 8:31 doi:10.1186/1471-2121-8-31