Additional File 1.
Determination of the specificity of EHD peptide antisera. HEK 293FT cells in 100-mm tissue culture dishes were transiently transfected with DNA encoding a single EHD-GFP (6 μg) construct. Cell lysates were prepared as in Methods. Aliquots of 100 μg were loaded onto an 8% SDS-PAGE gel, transferred to a PVDF membrane, and immunoblotted with specific EHD anti-sera as shown. Relative molecular weight (MW) markers are indicated in kiloDaltons (kD).
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George et al. BMC Cell Biology 2007 8:3 doi:10.1186/1471-2121-8-3